mouse anti alpha sarcomeric actin α sma Search Results


human/mouse/rat alpha-smooth muscle actin antibody  (Bio-Techne corporation)
99
Bio-Techne corporation human/mouse/rat alpha-smooth muscle actin antibody
Human/Mouse/Rat Alpha Smooth Muscle Actin Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human/mouse/rat alpha-smooth muscle actin antibody/product/Bio-Techne corporation
Average 99 stars, based on 1 article reviews
human/mouse/rat alpha-smooth muscle actin antibody - by Bioz Stars, 2026-03
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fitc-conjugated mouse monoclonal anti-α-smooth muscle actin (α sma)  (Rockland Immunochemicals)
90
Rockland Immunochemicals fitc-conjugated mouse monoclonal anti-α-smooth muscle actin (α sma)
Fitc Conjugated Mouse Monoclonal Anti α Smooth Muscle Actin (α Sma), supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-conjugated mouse monoclonal anti-α-smooth muscle actin (α sma)/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
fitc-conjugated mouse monoclonal anti-α-smooth muscle actin (α sma) - by Bioz Stars, 2026-03
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mouse anti-α-actin, smooth muscle (α-sma)  (Cell Marque)
90
Cell Marque mouse anti-α-actin, smooth muscle (α-sma)
Mouse Anti α Actin, Smooth Muscle (α Sma), supplied by Cell Marque, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-α-actin, smooth muscle (α-sma)/product/Cell Marque
Average 90 stars, based on 1 article reviews
mouse anti-α-actin, smooth muscle (α-sma) - by Bioz Stars, 2026-03
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hrp-mouse monoclonal anti-tgf-β1, α-sma and β-actin  (Beijing CWBio)
90
Beijing CWBio hrp-mouse monoclonal anti-tgf-β1, α-sma and β-actin
Hrp Mouse Monoclonal Anti Tgf β1, α Sma And β Actin, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp-mouse monoclonal anti-tgf-β1, α-sma and β-actin/product/Beijing CWBio
Average 90 stars, based on 1 article reviews
hrp-mouse monoclonal anti-tgf-β1, α-sma and β-actin - by Bioz Stars, 2026-03
90/100 stars
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mouse anti-alpha-smooth muscle actin (α-sma)-cy3  (Merck & Co)
90
Merck & Co mouse anti-alpha-smooth muscle actin (α-sma)-cy3
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Mouse Anti Alpha Smooth Muscle Actin (α Sma) Cy3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-alpha-smooth muscle actin (α-sma)-cy3/product/Merck & Co
Average 90 stars, based on 1 article reviews
mouse anti-alpha-smooth muscle actin (α-sma)-cy3 - by Bioz Stars, 2026-03
90/100 stars
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mouse anti–smooth muscle α-actin (sma) monoclonal antibody  (Affinity Biosciences)
90
Affinity Biosciences mouse anti–smooth muscle α-actin (sma) monoclonal antibody
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Mouse Anti–Smooth Muscle α Actin (Sma) Monoclonal Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti–smooth muscle α-actin (sma) monoclonal antibody/product/Affinity Biosciences
Average 90 stars, based on 1 article reviews
mouse anti–smooth muscle α-actin (sma) monoclonal antibody - by Bioz Stars, 2026-03
90/100 stars
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mouse anti-α-smooth muscle actin  (Becton Dickinson)
90
Becton Dickinson mouse anti-α-smooth muscle actin
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Mouse Anti α Smooth Muscle Actin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-α-smooth muscle actin/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-α-smooth muscle actin - by Bioz Stars, 2026-03
90/100 stars
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mouse anti-α-smooth muscle actin (α-sma)  (Beyotime)
90
Beyotime mouse anti-α-smooth muscle actin (α-sma)
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Mouse Anti α Smooth Muscle Actin (α Sma), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-α-smooth muscle actin (α-sma)/product/Beyotime
Average 90 stars, based on 1 article reviews
mouse anti-α-smooth muscle actin (α-sma) - by Bioz Stars, 2026-03
90/100 stars
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alpha-smooth muscle actin antibody (1a4/asm-1) - bsa free  (Bio-Techne corporation)
94
Bio-Techne corporation alpha-smooth muscle actin antibody (1a4/asm-1) - bsa free
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Alpha Smooth Muscle Actin Antibody (1a4/Asm 1) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alpha-smooth muscle actin antibody (1a4/asm-1) - bsa free/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
alpha-smooth muscle actin antibody (1a4/asm-1) - bsa free - by Bioz Stars, 2026-03
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mouse monoclonal anti-α-smooth muscle actin (α-sma)  (Promega)
90
Promega mouse monoclonal anti-α-smooth muscle actin (α-sma)
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Mouse Monoclonal Anti α Smooth Muscle Actin (α Sma), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-α-smooth muscle actin (α-sma)/product/Promega
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-α-smooth muscle actin (α-sma) - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-mouse antibodies specific for α-smooth muscle actin (α-sma), integrin β1 and phosphorylated akt (p-akt)  (Abbkine Inc)
90
Abbkine Inc rabbit anti-mouse antibodies specific for α-smooth muscle actin (α-sma), integrin β1 and phosphorylated akt (p-akt)
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Rabbit Anti Mouse Antibodies Specific For α Smooth Muscle Actin (α Sma), Integrin β1 And Phosphorylated Akt (P Akt), supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse antibodies specific for α-smooth muscle actin (α-sma), integrin β1 and phosphorylated akt (p-akt)/product/Abbkine Inc
Average 90 stars, based on 1 article reviews
rabbit anti-mouse antibodies specific for α-smooth muscle actin (α-sma), integrin β1 and phosphorylated akt (p-akt) - by Bioz Stars, 2026-03
90/100 stars
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alpha-smooth muscle actin antibody (spm332)  (Bio-Techne corporation)
92
Bio-Techne corporation alpha-smooth muscle actin antibody (spm332)
Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers <t>α-smooth</t> <t>muscle</t> <t>actin</t> <t>(α-SMA)</t> or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.
Alpha Smooth Muscle Actin Antibody (Spm332), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alpha-smooth muscle actin antibody (spm332)/product/Bio-Techne corporation
Average 92 stars, based on 1 article reviews
alpha-smooth muscle actin antibody (spm332) - by Bioz Stars, 2026-03
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Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers α-smooth muscle actin (α-SMA) or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.

Journal: Neoplasia (New York, N.Y.)

Article Title: Inhibition of fibronectin accumulation suppresses TUMOR growth

doi: 10.1016/j.neo.2021.06.012

Figure Lengend Snippet: Changes in fibronectin affect blood vessels and proliferation. (A) Tumor lesions exhibit less fibronectin staining in sections from knockdown (Kd) tumors compared to controls (CT). Bars represent 100 μm. (B) Kd tumors contain less fibronectin as determined by ELISA. Pieces obtained from tumor samples were weighed and lysed in protein lysis buffer to evaluate by ELISA. The amount of fibronectin was corrected to total protein measured by the BCA method. N=3/6 replicates. (C) Expression of fibronectin mRNA originating from the cancer cells (human) and the host (murine) was diminished. Using primers specific for human and murine fibronectin it was possible to differentiate between both in qPCR analyses. Human fibronectin was corrected to human HPRT and murine fibronectin to murine β-actin. N=5/3 and 7/5 for the two graphs (left to right). (D) The area of CD31 + blood vessels diminishes as does the number of vessels stained with both CD31 (in red) and the pericyte markers α-smooth muscle actin (α-SMA) or desmin (in green). Examples are shown. Bars represent 100 μm. N=11/10 for CD31 + area and stainings with αSMA and 7/7 for stainings with desmin. (E) Endothelial cells from knockdown tumors produce less fibronectin. Isolated endothelial cells were cultured in the presence of fibronectin-depleted FCS and fibronectin evaluated in the conditioned media by ELISA. N=11/5. (F) Proliferation in tumor sections was diminished in Kd tumors as evidenced by the decrease in the percentage of Ki67 + cells. N=9/9. Examples are shown. Bars represent 100 μm. (G) Tumor sections were stained using TUNEL to mark apoptotic cells. No difference was seen between CT and Kd tumors. N=3/3. Examples are shown. Bars represent 100 μm. All pairs were evaluated using t-test. * P <0.05, ** P <0.005, *** P <0.0001.

Article Snippet: We also used rat anti-mouse CD31 (BIO-RAD; MCA2388GA; 1:100), mouse anti-alpha-smooth muscle actin (α-SMA)-Cy3 (Merck; #A2547; 1:200), rabbit anti-desmin (Dianova; #DLN-13732; 1:100), rabbit monoclonal antibody against YAP (Cell signaling #14074, 1:200), goat anti-rabbit-Alexa 647 (Abcam #ab150079; 1:1000), goat anti-mouse-Cy2 (Dianova; #115-225-166; 1:1000), goat anti-rat-Alexa 594 (Dianova; #112-585-062; 1:1000).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Lysis, Expressing, Isolation, Cell Culture, TUNEL Assay

Histology of pUR4-treated tumors confirms changes in matrix and reveals a decrease in proliferation. (A) Stained tumor sections show a decrease in fibronectin and collagen in pUR4-treated animals and no changes in R1R2-treated animals. Bars represent 100 μm. (B) Western blots from the tumors confirm a decrease in fibronectin and collagen with pUR4 treatment, but collagen I did not decrease with R1R2 therapy. N=4/4/4/4 replicates/treatment. Relevant pairs were evaluated by t-tests. * P <0.05. (C) Proliferation was diminished after treatment with pUR4. Sections were stained for Ki67. Bars represent 100 μm. N=9/9/9/9. Pairs were evaluated by t-tests. ** P <0.01. (D) Apoptosis was not affected in vivo by the various treatments. Sections were stained for TUNEL. Examples are shown. Bars represent 100 μm. N=9/9/9/9. Pairs were evaluated by t-tests. (E) Evaluation of blood vessels shows a decrease in area of CD31 + vessels with pUR4 treatment as well as the number of CD31 + αSMA + or CD31 + desmin + double positive vessels (adjusted to the area). Sections from representative tumors with bars representing 100μm. These are intratibial tumors from mice treated for 10 days with the peptides. N=12/12/11/12 for CD31 alone or with α-smooth muscle actin (αSMA) and 11/10/7/7 for staining with desmin. Pairs were compared by t-test. * P <0.05, **** P <0.0001.

Journal: Neoplasia (New York, N.Y.)

Article Title: Inhibition of fibronectin accumulation suppresses TUMOR growth

doi: 10.1016/j.neo.2021.06.012

Figure Lengend Snippet: Histology of pUR4-treated tumors confirms changes in matrix and reveals a decrease in proliferation. (A) Stained tumor sections show a decrease in fibronectin and collagen in pUR4-treated animals and no changes in R1R2-treated animals. Bars represent 100 μm. (B) Western blots from the tumors confirm a decrease in fibronectin and collagen with pUR4 treatment, but collagen I did not decrease with R1R2 therapy. N=4/4/4/4 replicates/treatment. Relevant pairs were evaluated by t-tests. * P <0.05. (C) Proliferation was diminished after treatment with pUR4. Sections were stained for Ki67. Bars represent 100 μm. N=9/9/9/9. Pairs were evaluated by t-tests. ** P <0.01. (D) Apoptosis was not affected in vivo by the various treatments. Sections were stained for TUNEL. Examples are shown. Bars represent 100 μm. N=9/9/9/9. Pairs were evaluated by t-tests. (E) Evaluation of blood vessels shows a decrease in area of CD31 + vessels with pUR4 treatment as well as the number of CD31 + αSMA + or CD31 + desmin + double positive vessels (adjusted to the area). Sections from representative tumors with bars representing 100μm. These are intratibial tumors from mice treated for 10 days with the peptides. N=12/12/11/12 for CD31 alone or with α-smooth muscle actin (αSMA) and 11/10/7/7 for staining with desmin. Pairs were compared by t-test. * P <0.05, **** P <0.0001.

Article Snippet: We also used rat anti-mouse CD31 (BIO-RAD; MCA2388GA; 1:100), mouse anti-alpha-smooth muscle actin (α-SMA)-Cy3 (Merck; #A2547; 1:200), rabbit anti-desmin (Dianova; #DLN-13732; 1:100), rabbit monoclonal antibody against YAP (Cell signaling #14074, 1:200), goat anti-rabbit-Alexa 647 (Abcam #ab150079; 1:1000), goat anti-mouse-Cy2 (Dianova; #115-225-166; 1:1000), goat anti-rat-Alexa 594 (Dianova; #112-585-062; 1:1000).

Techniques: Staining, Western Blot, In Vivo, TUNEL Assay